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Q1
What is the price of TSI Agar HiMedia (500g) in Bangladesh?
Triple Sugar Iron Agar (TSI Agar HiMedia) - Differential Media
৳7,000
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Product Code : P0404
Product Specification
Key product information at a glance.
Product Code
P0404
Brand
Himedia
Origin
Made in india
Category
Lab Chemicals & Reagents
Subcategory
Lab Chemicals
Available Sizes
500gm
Availability
In Stock
Product
Triple Sugar Iron Agar (TSI Agar - Dehydrated Differential Media)
Form
Homogeneous, free-flowing pinkish-beige powder
Quantity Required
64.75 g/L of purified or distilled water
Typical Formula (g/L)
Peptone: 20.0 | HM Peptone B: 3.0 | Yeast extract: 3.0 | Lactose: 10.0 | Sucrose: 10.0 | Dextrose (Glucose): 1.0 | Ferric citrate: 0.3 | Sodium chloride: 5.0 | Sodium thiosulphate: 0.3 | Phenol red: 0.024 | Agar: 12.0
Final pH (at 25°C)
7.4 ± 0.2
Sterilization Parameter
Autoclave at 121°C (15 lbs pressure) for 15 minutes; cool to form slants with deep butts
Storage
Store dehydrated powder tightly capped between 10°C and 30°C in a dry place
Pack Size
500 grams HDPE container
Product Description
Triple Sugar Iron Agar (TSI) | Differential Culture Medium
Triple Sugar Iron Agar (TSI Agar, HiMedia Code: M021) is one of the most powerful and widely utilized differential culture media in diagnostic bacteriology. Designed specifically to evaluate the fermentation of glucose, lactose, and sucrose, alongside gas production and hydrogen sulfide ($H_2S$) generation, TSI Agar plays a vital role in identifying and classifying enteric Gram-negative bacilli (Enterobacteriaceae) from clinical specimens, food products, and environmental samples. It is an indispensable diagnostic reagent across hospital pathology departments, public health laboratories, pharmaceutical testing units, and university microbiology faculties throughout Bangladesh.
Identifying enteric pathogens requires a multi-parameter biochemical test capable of distinguishing non-pathogenic coliforms from virulent organisms like Salmonella and Shigella species. TSI Agar is prepared as an agar slant with a deep butt, creating both aerobic (slant) and anaerobic (butt) microenvironments. It contains 0.1% glucose, 1.0% lactose, and 1.0% sucrose, combined with phenol red pH indicator and ferrous sulfate. When inoculated bacteria ferment glucose (present in lower concentration), the entire medium initially turns yellow due to acid production. Once glucose is exhausted, aerobic bacteria on the slant oxidize organic acids, turning the slant red while the anaerobic butt remains acidic (yellow). If lactose or sucrose is also fermented, both the slant and butt remain yellow due to heavy acid accumulation. Gas production is visualized through cracks or bubbles in the agar, while $H_2S$ production reacts with ferrous sulfate to form a distinct black precipitate.
Supplied as a free-flowing, homogeneous pinkish-beige dehydrated powder, it dissolves efficiently in distilled water (typically 64.75 g/L), is distributed into tubes, and autoclaved to solidify as deep butts with slants. Victor Scientific Co. supplies authentic HiMedia culture media, analytical reagents, and laboratory apparatus nationwide with batch traceability and dependable delivery across all 64 districts of Bangladesh.
✹ Key Features
Authentic HiMedia Quality (Code: M021)
Manufactured to stringent international microbiological benchmarks, ensuring consistent carbohydrate ratios and sharp color transition boundaries.
Multi-Parameter Enteric Differentiation
Simultaneously evaluates three sugars (glucose, lactose, sucrose), gas formation, and hydrogen sulfide ($H_2S$) production in a single tube.
Dual Aerobic/Anaerobic Slant-Butt Design
Provides both aerobic slant and anaerobic butt zones to detect oxidative versus fermentative metabolic pathways.
Clear Phenol Red pH Indicator
Shifts visibly from red to yellow under acidic conditions and back to red under alkaline oxidative conditions.
Hydrogen Sulfide ($H_2S$) Detection
Incorporates sodium thiosulfate and ferrous sulfate to reveal $H_2S$-producing organisms through brilliant black iron sulfide precipitation.
Convenient Preparation Ratio
Requires suspending 64.75 grams in 1,000 ml of purified distilled water, boiling to dissolve completely, distributing into tubes, and autoclaving.
✹ Applications
- Identification and differentiation of Enterobacteriaceae in clinical diagnostic and hospital pathology laboratories
- Confirmatory biochemical screening for Salmonella and Shigella isolation workflows
- Food, dairy, and beverage hygiene quality control and enteric pathogen surveillance
- Public health municipal water testing and sewage effluent monitoring
- Academic undergraduate and postgraduate laboratory practicals in medical microbiology and bacteriology
✹ Why Choose HiMedia TSI Agar?
- Globally recognized gold-standard formulation for rapid presumptive biochemical profiling of enteric Gram-negative bacilli.
- Combines multiple diagnostic tests into a single tube, reducing reagent consumption and procedural complexity.
- Highly soluble powder disperses smoothly into solution during laboratory preparation.
- Genuine institutional procurement and dependable stock availability backed by Victor Scientific Co.
✹ Benefits
- Accelerates clinical diagnostic turnaround times by providing definitive metabolic data within 18 to 24 hours.
- Maximizes laboratory cost-efficiency through high-yield 500g packaging capable of producing nearly 7.5 liters of finished testing media.
- Reduces procedural errors with certified formulation standards and clear technical documentation.
- Backed by dependable nationwide distribution and technical support through Victor Scientific Co.
✹ About Victor Scientific Co.
Victor Scientific Co. provides quality laboratory equipment, measuring instruments and scientific tools in Bangladesh. We supply reliable products for educational institutions, industries, laboratories and professional users.
Frequently Asked Questions (FAQ)
A1
The price of Triple Sugar Iron Agar HiMedia M021 (500g pack) in Bangladesh typically ranges between BDT 7,500 and BDT 9,800, depending on import batches and foreign exchange rates. Contact Victor Scientific Co. directly for exact institutional quotes and bulk availability.
Q2
How do you interpret a "Red Slant / Yellow Butt" (K/A) reaction on TSI Agar?
A2
A red slant over a yellow butt (alkaline slant / acidic butt) indicates that the organism fermented glucose only. Because glucose concentration is low (0.1%), it is quickly depleted, after which the bacteria oxidize amino acids on the aerobic slant, producing an alkaline red reaction while the anaerobic butt remains acidic yellow.
Q3
What does a black precipitate in the tube indicate?
A3
The black precipitate indicates hydrogen sulfide ($H_2S$) production. Organisms reduce sodium thiosulfate to hydrogen sulfide gas, which reacts with ferrous sulfate in the medium to form insoluble black ferrous sulfide (typically seen in Salmonella species).
Q4
What is the preparation ratio for TSI Agar powder?
A4
Suspend 64.75 grams of dehydrated powder in 1,000 ml of purified distilled water, heat with frequent agitation to boiling to dissolve completely, distribute into test tubes, and autoclave at 15 psi (121°C) for 15 minutes. Cool in a slanted position to ensure a deep butt and adequate slant surface area.
Q5
Does Victor Scientific Co. deliver culture media safely across Bangladesh?
A5
Yes, Victor Scientific Co. packages all culture media bottles in protective cushioning and moisture-barrier cartons, ensuring rapid courier delivery to all 64 districts in Bangladesh—including Chattogram, Rajshahi, Khulna, and Sylhet—within 24 to 48 hours.
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