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Q1
What is the price of EMB Agar HiMedia (500g) in Bangladesh?
EMB Agar (Eosin Methylene Blue Agar) HiMedia
৳8,500
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Product Code : P0408
Product Specification
Key product information at a glance.
Product Code
P0408
Brand
Himedia
Origin
Made in india
Category
Lab Chemicals & Reagents
Subcategory
Lab Chemicals
Available Sizes
500gm
Availability
In Stock
Product
EMB Agar (Eosin Methylene Blue Agar, Levine) - Dehydrated Media
Form
Homogeneous, free-flowing purple powder
Quantity Required
37.50 g/L of purified or distilled water
Typical Formula (g/L)
Peptone: 10.0 | Lactose: 5.0 | Sucrose: 5.0 | Dipotassium hydrogen phosphate: 2.0 | Eosin Y: 0.4 | Methylene blue: 0.065 | Agar: 13.5
Final pH (at 25°C)
7.1 ± 0.2
Sterilization Parameter
Autoclave at 121°C (15 lbs pressure) for 15 minutes
Storage
Store dehydrated powder tightly capped between 10°C and 30°C in a dry place
Pack Size
500 grams HDPE container
Product Description
EMB Agar (Eosin Methylene Blue) HiMedia
EMB Agar (Eosin Methylene Blue Agar, Levine formulation, HiMedia Code: M317) is a classic differential and selective growth medium widely utilized in diagnostic bacteriology, water quality surveillance, and food microbiology laboratories. Engineered primarily for the isolation and presumptive identification of enteric Gram-negative bacilli—including coliforms and pathogenic non-lactose fermenters—from clinical specimens, dairy goods, and municipal water supplies, it serves as an essential reagent across hospital pathology departments, public health testing facilities, and university microbiology faculties throughout Bangladesh.
Differentiating fecal coliforms from non-pathogenic enteric organisms requires a selective chemical matrix that inhibits Gram-positive bacteria while highlighting carbohydrate fermentation pathways. EMB Agar achieves selective suppression using the dyes eosin Y and methylene blue, which inhibit the growth of Gram-positive cocci and bacilli without impairing Gram-negative recovery. Simultaneously, it provides differential diagnostics via lactose and sucrose incorporation. Lactose-fermenting coliforms produce acid end-products that precipitate the eosin-methylene blue complex; rapid lactose fermenters like Escherichia coli yield dark-centered colonies with a characteristic greenish-metallic sheen. Other slow or less vigorous lactose fermenters (such as Enterobacter and Klebsiella) produce larger, mucoid pink-to-purple colonies, whereas non-lactose fermenters (such as Salmonella and Shigella) appear translucent, colorless, or pale pink.
Supplied as a free-flowing, homogeneous purple dehydrated powder, it dissolves efficiently in distilled water (typically 37.5 g/L) and autoclaves to a dark purple opalescent gel with a greenish cast. Victor Scientific Co. supplies authentic HiMedia culture media, analytical reagents, and laboratory apparatus nationwide with batch traceability and dependable delivery across all 64 districts of Bangladesh.
✹ Key Features
Authentic HiMedia Quality (Code: M317)
Manufactured to stringent international Levine formulation benchmarks, ensuring consistent dye inhibition and sharp metallic sheen differentiation.
Selective Gram-Negative Suppression
Contains eosin Y and methylene blue dyes to selectively inhibit Gram-positive background flora.
Classic Metallic Sheen Differentiation
Rapid lactose/sucrose fermenters like E. coli precipitate the dyes to exhibit a striking green metallic sheen under reflected light.
Dual Carbohydrate System
Incorporates both lactose and sucrose to capture a broader spectrum of coliform fermenters.
Convenient Preparation Ratio
Requires suspending 37.5 grams in 1,000 ml of purified distilled water, boiling to dissolve completely, and autoclaving at 121°C for 15 minutes.
Moisture-Resistant HDPE Packaging
Packaged in a 500-gram hermetically sealed container designed to safeguard the dehydrated powder against ambient humidity and degradation.
✹ Applications
- Clinical microbiological screening of stool specimens and urine cultures for enteric pathogens
- Municipal drinking water safety testing, fecal coliform enumeration, and wastewater surveillance
- Food and dairy product hygiene testing, bioburden monitoring, and sanitary verification
- Pharmaceutical microbial limit testing and quality assurance validation
- Academic undergraduate and postgraduate laboratory practicals in medical microbiology and bacteriology
✹ Why Choose HiMedia EMB Agar?
- Globally recognized Levine formula trusted by clinical microbiologists and water testing authorities for reliable E. coli identification.
- Distinct colonial morphology (especially the green metallic sheen) enables rapid presumptive screening without complex biochemical testing.
- Highly soluble powder disperses smoothly into solution during preparation.
- Genuine institutional procurement and dependable stock availability backed by Victor Scientific Co.
✹ Benefits
- Accelerates diagnostic workflows by combining selective isolation and visual differentiation on a single agar plate.
- Maximizes laboratory cost-efficiency through high-yield 500g packaging capable of producing over 13 liters of finished testing media.
- Reduces procedural errors with certified formulation standards and clear technical documentation.
- Backed by dependable nationwide distribution and technical support through Victor Scientific Co.
✹ About Victor Scientific Co.
Victor Scientific Co. provides quality laboratory equipment, measuring instruments and scientific tools in Bangladesh. We supply reliable products for educational institutions, industries, laboratories and professional users.
Frequently Asked Questions (FAQ)
A1
The price of EMB Agar HiMedia M317 (500g pack) in Bangladesh typically ranges between BDT 7,500 and BDT 9,800, depending on import batch and exchange rates. Contact Victor Scientific Co. directly for exact institutional quotes and bulk availability.
Q2
What causes the green metallic sheen on EMB agar?
A2
The metallic sheen is produced by Escherichia coli and certain other vigorous lactose-fermenting coliforms when acid production drops the local pH, causing a precipitation of the eosin Y and methylene blue dye complex onto the colony surface.
Q3
How do non-lactose fermenters appear on EMB Agar?
A3
Non-lactose fermenting bacteria (such as Salmonella and Shigella species) do not produce acid from lactose or sucrose; consequently, their colonies remain colorless, translucent, or pale pink, blending into the background.
Q4
What is the preparation ratio for EMB Agar powder?
A4
Suspend 37.5 grams of dehydrated powder in 1,000 ml of purified distilled water, heat with frequent agitation to dissolve completely, and autoclave at 15 psi (121°C) for 15 minutes. Swirl well before pouring to ensure the suspended dye is evenly distributed.
Q5
Does Victor Scientific Co. deliver culture media safely across Bangladesh?
A5
Yes, Victor Scientific Co. packages all culture media bottles in protective cushioning and moisture-barrier cartons, ensuring rapid courier delivery to all 64 districts in Bangladesh—including Chattogram, Rajshahi, Khulna, and Sylhet—within 24 to 48 hours.
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