C.L.E.D. Agar w/ Bromo Thymol Blue (HiMedia) - 500g

Name: C.L.E.D. Agar w/ Bromo Thymol Blue (HiMedia) - 500g
Brand: Himedia
SKU: P0409
Price: 7500 BDT
Availability: InStock

৳7,500

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Product Code : P0409

Size -

500gm

Brand : Himedia

Origin : Made in india

Capacity : 500gm

In Stock

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Product Specification

Key product information at a glance.

Product Code

P0409

Brand

Himedia

Origin

Made in india

Capacity

500gm

Product Description

C.L.E.D. Agar (Cysteine Lactose Electrolyte Deficient) HiMedia

C.L.E.D. Agar with Bromo Thymol Blue (HiMedia Code: M792) is a premier differential and diagnostic culture medium tailored specifically for urine bacteriology and urinary tract infection (UTI) screening. Developed to address the challenges of routine urine cultures—such as the overgrowth and swarming of Proteus species that obscure other pathogens—C.L.E.D. medium restricts electrolyte concentration to maintain discrete, countable colonies. It serves as an indispensable diagnostic staple across hospital pathology units, medical diagnostic labs, clinical microbiology facilities, and university faculties throughout Bangladesh.

Cultivating fragile urinary pathogens and assessing quantitative bacteriuria requires a reliable formulation that prevents colonial merging while offering sharp biochemical differentiation. C.L.E.D. Agar achieves electrolyte deficiency to inhibit the characteristic swarming motility of Proteus species without requiring toxic chemical additives. It incorporates peptone, tryptone, and HM peptone B to supply essential amino acids, nitrogenous nutrients, and vitamins, alongside lactose for fermentation testing. L-cystine is added to promote the growth of cystine-dependent "dwarf" coliform colonies that might otherwise fail to develop. The bromothymol blue pH indicator shifts from green to bright yellow in acidic conditions produced by lactose fermentation, allowing effortless visual differentiation of lactose-fermenting pathogens (such as Escherichia coli) from non-fermenters.

Supplied as a free-flowing, homogeneous cream-to-yellow dehydrated powder, it dissolves efficiently in distilled water (typically 36.15 g/L) and autoclaves to a clear, green-colored gel. Victor Scientific Co. supplies authentic HiMedia culture media, analytical reagents, and laboratory apparatus nationwide with batch traceability and dependable delivery across all 64 districts of Bangladesh.

✹ Key Features

Authentic HiMedia Quality (Code: M792)

Manufactured to strict international diagnostic benchmarks, ensuring consistent electrolyte restriction and sharp color-change differentiation.

Swarming Suppression of Proteus Species

Electrolyte-deficient matrix successfully inhibits the swarming motility of Proteus strains, allowing isolated colony enumeration.

Lactose Fermentation Differentiation

Incorporates bromothymol blue indicator shifting from green to yellow in response to acid production by lactose fermenters.

Cystine-Enriched Formula

Contains L-cystine to support the robust development of cystine-dependent dwarf coliform colonies.

Convenient Preparation Ratio

Requires suspending 36.15 grams in 1,000 ml of purified distilled water, boiling to dissolve completely, and autoclaving at 121°C for 15 minutes.

Moisture-Resistant HDPE Packaging

Packaged in a 500-gram hermetically sealed container designed to safeguard the dehydrated powder against ambient humidity and degradation.

✹ Applications

- Clinical diagnosis and quantitative bacteruria counts for urinary tract infections (UTIs) from urine samples

- Isolation and presumptive identification of common urinary pathogens (E. coli, Klebsiella, Proteus, Enterococcus, Pseudomonas)

- Dip-stick procedures and dip-inoculum transport systems for field urine collection

- Hospital pathology and clinical microbiology quality control routines

- Academic undergraduate and postgraduate laboratory practicals in medical bacteriology and clinical diagnostics

✹ Why Choose HiMedia C.L.E.D. Agar?

- Globally trusted formulation standard in clinical urology for clear isolation and counting of mixed urinary flora.

- Eliminates swarming interference, preventing obscured pathogens and inaccurate colony counts.

- Highly soluble powder disperses smoothly into solution during preparation.

- Genuine institutional procurement and dependable stock availability backed by Victor Scientific Co.

✹ Benefits

- Accelerates clinical diagnostic workflows by enabling simultaneous isolation, counting, and identification on a single plate.

- Maximizes laboratory cost-efficiency through high-yield 500g packaging capable of producing nearly 14 liters of finished testing media.

- Reduces procedural errors with certified formulation standards and clear technical documentation.

- Backed by dependable nationwide distribution and technical support through Victor Scientific Co.

✹ About Victor Scientific Co.

Victor Scientific Co. provides quality laboratory equipment, measuring instruments and scientific tools in Bangladesh. We supply reliable products for educational institutions, industries, laboratories and professional users.

Frequently Asked Questions (FAQ)

Q1

What is the price of C.L.E.D. Agar HiMedia (500g) in Bangladesh?

A1

The price of C.L.E.D. Agar HiMedia M792 (500g pack) in Bangladesh typically ranges between BDT 7,500 and BDT 9,500, depending on batch import costs and foreign exchange rates. Contact Victor Scientific Co. directly for exact institutional quotes and bulk availability.

Q2

Why is the medium called "Electrolyte Deficient"?

A2

Electrolytes (such as salt ions) are restricted in this medium specifically to prevent the swarming behavior of Proteus species, which would otherwise overrun the agar surface and obscure other bacterial colonies during a urine culture.

Q3

How do Escherichia coli colonies appear on C.L.E.D. Agar?

A3

Escherichia coli ferments lactose, producing acid that lowers the pH and turns the bromothymol blue indicator yellow, typically forming yellow, opaque colonies with a deeper yellow center.

Q4

What is the preparation ratio for C.L.E.D. Agar powder?

A4

Suspend 36.15 grams of dehydrated powder in 1,000 ml of purified distilled water, heat with frequent agitation to dissolve completely, and autoclave at 15 psi (121°C) for 15 minutes.

Q5

Does Victor Scientific Co. deliver culture media safely across Bangladesh?

A5

Yes, Victor Scientific Co. packages all culture media bottles in protective cushioning and moisture-barrier cartons, ensuring rapid courier delivery to all 64 districts in Bangladesh—including Chattogram, Rajshahi, Khulna, and Sylhet—within 24 to 48 hours.