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Q1
What is the price of HiCrome Chromogenic Coliform Agar HiMedia (500g) in Bangladesh?
HiCrome Chromogenic Coliform Agar (CCA / HiCrome M1991I) - 500g
৳15,000
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Product Code : P0410
Product Specification
Key product information at a glance.
Product Code
P0410
Brand
Himedia
Origin
Made in india
Capacity
500gm
Category
Lab Chemicals & Reagents
Subcategory
Lab Chemicals
Available Sizes
100gm
Availability
In Stock
Product
HiCrome Chromogenic Coliform Agar (CCA Agar / ISO 9308-1)
Form
Homogeneous, free-flowing cream to yellow powder
Quantity Required
30.92 g/L of purified or distilled water
Typical Formula (g/L)
Tryptone: 1.0 | Yeast Extract: 2.0 | NaCl: 5.0 | Sodium dihydrogen phosphate: 2.2 | Disodium hydrogen phosphate: 2.7 | Sodium pyruvate: 1.0 | Sorbitol: 1.0 | Tryptophan: 1.0 | Tergitol-7: 0.15 | Chromogenic mix (X-gal, X-glucuronide, IPTG): Approx. 0.4 | Agar: 15.0
Final pH (at 25°C)
6.8 ± 0.2
Sterilization Parameter
Heat to boiling to dissolve completely; DO NOT AUTOCLAVE
Storage
Store dehydrated powder tightly capped between 15°C and 25°C in a dry place
Pack Size
500 grams HDPE container
Product Description
HiCrome Chromogenic Coliform Agar HiMedia
HiCrome Chromogenic Coliform Agar (CCA Agar, HiMedia Code: M1991I) is an advanced, high-performance dehydrated culture medium manufactured in strict accordance with ISO 9308-1:2014 specifications. Designed for water quality testing, environmental monitoring, and food safety compliance, this chromogenic medium allows laboratories to simultaneously detect and differentiate Escherichia coli and coliform bacteria directly on a single selective plate. It is an essential diagnostic reagent across public health laboratories, municipal water treatment plants, beverage manufacturing units, and university microbiology faculties throughout Bangladesh.
传统 detection methods for coliforms and E. coli involve tedious multi-stage biochemical confirmatory tests (such as presumptive, confirmed, and completed lactose fermentation tests). HiCrome Chromogenic Coliform Agar eliminates this delay by incorporating distinct chromogenic substrates that target specific bacterial enzymes. The enzyme beta-D-galactosidase, produced by total coliforms, cleaves 6-chloro-3-indoxyl-beta-D-galactopyranoside to form distinct pink-to-red colonies. Simultaneously, the enzyme beta-D-glucuronidase, specific to Escherichia coli, cleaves 5-bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, causing E. coli colonies to appear dark blue to violet. Tergitol-7 is included to selectively inhibit Gram-positive background flora, ensuring clean, interference-free recovery of target Gram-negative organisms.
Supplied as a free-flowing, homogeneous cream-to-yellow dehydrated powder, it dissolves efficiently in distilled water (typically 30.92 g/L). Per ISO specifications, the medium is brought to a boil to dissolve completely and **must not be autoclaved**, protecting the heat-sensitive chromogens. Victor Scientific Co. supplies authentic HiMedia chromogenic culture media, analytical reagents, and laboratory apparatus nationwide with batch-specific Certificates of Analysis (COA) and dependable delivery across all 64 districts of Bangladesh.
✹ Key Features
ISO 9308-1:2014 Compliant Formulation
Manufactured precisely to international standards for water quality testing and E. coli/coliform enumeration.
Dual Chromogenic Enzyme Differentiation
Contains specialized substrates targeting beta-galactosidase (pink/red coliforms) and beta-glucuronidase (dark blue/violet E. coli).
Built-In Selective System
Incorporates tergitol-7 to inhibit Gram-positive bacteria and unwanted background flora without suppressing target coliform recovery.
Rapid 21-Hour Diagnostic Readout
Delivers clear, highly readable colonial coloration after incubation at 36°C for 21 ± 3 hours.
Convenient Preparation Ratio
Requires suspending 30.92 grams in 1,000 ml of purified distilled water, heating to dissolve completely (**Do Not Autoclave**).
Moisture-Resistant HDPE Packaging
Packaged in a 500-gram hermetically sealed container designed to safeguard the dehydrated powder against ambient humidity and degradation.
✹ Applications
- Municipal drinking water safety testing, borehole surveillance, and wastewater compliance monitoring
- Bottled mineral water and soft drink quality assurance hygiene audits
- Food, dairy, and agricultural product microbiological safety screening
- Public health epidemiological investigations of water-borne outbreaks
- Academic undergraduate and postgraduate laboratory practicals in environmental microbiology and biotechnology
✹ Why Choose HiMedia HiCrome Coliform Agar?
- Eliminates the need for secondary biochemical confirmation steps (like indole or Kovacs tests for routine screening), saving valuable lab time.
- High specificity and distinct color contrast prevent misinterpretation of mixed microbial samples.
- Highly soluble powder disperses smoothly into solution during preparation.
- Genuine institutional procurement and dependable stock availability backed by Victor Scientific Co.
✹ Benefits
- Accelerates water testing turnaround times, enabling rapid public health reporting.
- Maximizes laboratory cost-efficiency through high-yield 500g packaging producing over 16 liters of finished testing media.
- Reduces procedural errors with certified ISO-compliant formulation standards and clear technical documentation.
- Backed by dependable nationwide distribution and technical support through Victor Scientific Co.
✹ About Victor Scientific Co.
Victor Scientific Co. provides quality laboratory equipment, measuring instruments and scientific tools in Bangladesh. We supply reliable products for educational institutions, industries, laboratories and professional users.
Frequently Asked Questions (FAQ)
A1
The price of HiCrome CCA Agar HiMedia M1991I (500g pack) in Bangladesh typically ranges between BDT 18,000 and BDT 24,000, reflecting specialized chromogenic reagent formulations and import costs. Contact Victor Scientific Co. directly for exact institutional quotes and bulk availability.
Q2
Should HiCrome Coliform Agar be autoclaved during preparation?
A2
No! This is critical: **Do Not Autoclave** or overheat HiCrome Coliform Agar. Autoclaving destroys the sensitive chromogenic substrates and IPTG additives. The medium is prepared by suspending the powder in water, bringing it to a boil with frequent agitation to dissolve completely, and then cooling directly to 45°C–50°C before pouring.
Q3
How do E. coli and other coliforms appear on this agar?
A3
Escherichia coli colonies appear dark blue to violet due to the cleavage of both chromogenic substrates. Other total coliforms (such as Citrobacter or Klebsiella species) appear pink to red. Non-coliform background bacteria are either inhibited or appear colorless.
Q4
Can a spot Kovacs test be performed on the colonies?
A4
Yes, to further confirm E. coli, a drop of Kovacs reagent can be applied directly to a dark blue/violet colony; the formation of a cherry-red color confirms a positive indole reaction.
Q5
Does Victor Scientific Co. deliver culture media safely across Bangladesh?
A5
Yes, Victor Scientific Co. packages all culture media bottles in protective cushioning and moisture-barrier cartons, ensuring rapid courier delivery to all 64 districts in Bangladesh—including Chattogram, Rajshahi, Khulna, and Sylhet—within 24 to 48 hours.
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