Mueller Hinton Agar (HA) | Antimicrobial Susceptibility Testing Medium
Mueller Hinton Agar (HiMedia Code: M173) is universally recognized as the gold standard, reference culture medium for performing Antimicrobial Susceptibility Testing (AST), particularly via the Kirby-Bauer disk diffusion method. Recommended by the Clinical and Laboratory Standards Institute (CLSI) and the World Health Organization (WHO), this specialized medium is indispensable for determining the efficacy of antibiotics and chemotherapeutic agents against pathogenic bacteria in clinical diagnostic laboratories, hospital pathology departments, pharmaceutical R&D units, and university microbiology faculties throughout Bangladesh.
Testing antibiotic efficacy requires a precisely balanced nutritional matrix that promotes rapid, uniform bacterial growth without inhibiting or antagonizing antimicrobial diffusion. Mueller Hinton Agar achieves this by utilizing an optimized blend of beef infusion, casein hydrolysate (providing rich amino acids and nitrogen), and starch. The starch component absorbs toxic metabolites produced by bacterial growth and prevents potential interference with antibiotic activity. Furthermore, its carefully controlled concentrations of divalent cations (calcium and magnesium) ensure accurate susceptibility profiling for aminoglycosides, tetracyclines, and fluoroquinolones against non-fastidious pathogens such as Pseudomonas, Staphylococcus, and Enterobacteriaceae.
Supplied as a free-flowing, homogeneous cream-to-yellow dehydrated powder, it dissolves efficiently in distilled water (typically 38.0 g/L) and autoclaves to a clear, pale-yellow agar that sets into a flat, uniform gel surface ideal for applying antibiotic disks. Victor Scientific Co. supplies authentic HiMedia culture media, analytical reagents, and laboratory apparatus nationwide with batch traceability and dependable delivery across all 64 districts of Bangladesh.
✹ Key Features
Authentic HiMedia Quality (Code: M173)
Manufactured to strict international CLSI and WHO standards, ensuring reproducible zone diameters and reliable antimicrobial resistance profiling.
Optimized Cation & Starch Formulation
Carefully regulated calcium/magnesium levels and incorporated starch prevent antibiotic antagonism and toxin accumulation.
Standardized Disk Diffusion Performance
Provides uniform gel depth and smooth surface texture, enabling optimal radial diffusion of antimicrobial susceptibility discs.
Convenient Preparation Ratio
Requires suspending 38.0 grams in 1,000 ml of purified distilled water, boiling to dissolve completely, and autoclaving at 121°C for 15 minutes.
Batch-Tested Quality Assurance
Tested rigorously with reference strains to guarantee consistent growth support and predictable inhibitory zone boundaries.
Moisture-Resistant HDPE Packaging
Packaged in a 500-gram hermetically sealed container designed to safeguard the dehydrated powder against ambient humidity and degradation.
✹ Applications
- Clinical antimicrobial susceptibility testing (AST) using Kirby-Bauer disk diffusion methods in hospital labs
- Determining minimum inhibitory concentrations (MIC) and resistance patterns of pathogenic clinical isolates
- Pharmaceutical quality control testing for antibiotic potency and antimicrobial compound screening
- Public health surveillance of emerging antibiotic-resistant bacterial strains (e.g., MRSA, MDR pathogens)
- Academic undergraduate and postgraduate laboratory practicals in medical microbiology and pharmacology
✹ Why Choose Mueller Hinton Agar?
- Globally mandated medium standard for clinical AST, ensuring compliance with international diagnostic guidelines.
- Eliminates batch variation, preventing false-resistant or false-susceptible diagnostic errors.
- Highly soluble powder dissolves smoothly without clumping, reducing preparation time in busy clinical settings.
- Genuine institutional procurement and dependable stock availability backed by Victor Scientific Co.
✹ Benefits
- Enhances diagnostic accuracy by maintaining strict biochemical parameters required for valid clinical reporting.
- Maximizes laboratory cost-efficiency through high-yield 500g packaging producing over 13 liters of finished testing agar.
- Reduces procedural errors with certified formulation standards and clear technical documentation.
- Backed by dependable nationwide distribution and technical support through Victor Scientific Co.
✹ About Victor Scientific Co.
Victor Scientific Co. provides quality laboratory equipment, measuring instruments and scientific tools in Bangladesh. We supply reliable products for educational institutions, industries, laboratories and professional users.