Blood Agar Base Himedia- Enriched Microbiological Culture Media 500g thumbnail 1

Blood Agar Base Himedia- Enriched Microbiological Culture Media 500g

৳7,500

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Product Code : P0400

Size -

Brand : Himedia

Origin : Made in india

In Stock
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Product Specification

Key product information at a glance.

Product Code
P0400
Brand
Himedia
Origin
Made in india
Category
Lab Chemicals & Reagents
Subcategory
Lab Chemicals
Available Sizes
500gm
Availability
In Stock
Product
Blood Agar Base (Dehydrated Culture Media)
Form
Homogeneous, free-flowing cream to yellow powder
Quantity Required
40.00 g/L of purified or distilled water
Typical Formula (g/L)
Beef heart infusion from: 500.0 | Tryptose: 10.0 | NaCl: 5.0 | Agar: 15.0
Final pH (at 25°C)
7.3 ± 0.2
Sterilization Parameter
Autoclave at 121°C (15 lbs pressure) for 15 minutes
Supplementation
Add 5%–10% sterile defibrinated blood after cooling to 45°C–50°C
Storage
Store dehydrated powder tightly capped between 10°C and 30°C in a dry place
Pack Size
500 grams HDPE container

Product Description

Blood Agar Base | Microbiology Culture Media

Blood Agar Base (HiMedia Code: M073, also known as Infusion Agar) is a premier general-purpose and enrichment growth medium widely used in clinical microbiology and research laboratories. Engineered to support the isolation, cultivation, and determination of hemolytic reaction patterns for demanding pathogens—such as Streptococcus species, Neisseria, Listeria, and Corynebacterium—it serves as an indispensable reagent across medical diagnostic centers, university pathology faculties, pharmaceutical quality control units, and public health laboratories throughout Bangladesh.

Cultivating fastidious microorganisms that refuse to grow on basic nutrient agars requires a rich infusion foundation containing complex animal proteins, amino acids, and growth factors. Blood Agar Base provides beef heart infusion and sensitive peptone digests as primary nutrient sources, combined with sodium chloride for osmotic balance and high-purity agar for stable gel formation (typically prepared at 40.0 g/L). When autoclaved, cooled to 45°C–50°C, and aseptically supplemented with 5% to 10% sterile defibrinated sheep, horse, or rabbit blood, it transforms into classic Blood Agar. This allows microbiologists to clearly observe diagnostic hemolysis patterns: beta-hemolysis (complete clear zones), alpha-hemolysis (partial green zones), and gamma-hemolysis (no change).

Supplied as a free-flowing, homogeneous cream-to-yellow dehydrated powder, it dissolves efficiently in purified water and resists clumping when stored in moisture-barrier containers. Victor Scientific Co. supplies authentic HiMedia culture media, analytical reagents, and laboratory apparatus nationwide with batch traceability and dependable delivery across all 64 districts of Bangladesh.

✹ Key Features
Authentic HiMedia Quality (Code: M073)
Manufactured to strict global microbiological benchmarks, ensuring consistent nutrient recovery, optimal gel firmness, and reproducible hemolysis reactions.

Rich Infusion-Based Formulation
Blends beef heart infusion solids, proteose peptone, and sodium chloride to provide a highly nutritious matrix for fastidious pathogens.

Clear Hemolytic Differentiation
When enriched with sterile blood, it clearly displays beta, alpha, and gamma hemolytic patterns essential for bacterial identification.

Versatile Basal Medium
Can be utilized unsupplemented as a general-purpose growth agar or fortified with blood, serum, or selective antibiotics for tailored diagnostic workflows.

Convenient Preparation Ratio
Requires suspending 40.0 grams in 1,000 ml of distilled water, dissolving by boiling, and autoclaving at 121°C for 15 minutes.

Moisture-Resistant HDPE Packaging
Packaged in a 500-gram hermetically sealed container designed to safeguard the dehydrated powder against ambient humidity and degradation.

✹ Applications
- Clinical diagnostic isolation and presumptive identification of streptococci, staphylococci, and other fastidious clinical pathogens
- Hemolytic activity evaluation (alpha, beta, and gamma hemolysis) in hospital pathology and diagnostic labs
- Pharmaceutical sterility testing, bioburden assessment, and microbial limit validation
- Food, dairy, and beverage quality control hygiene monitoring
- Academic undergraduate and postgraduate laboratory practicals in medical microbiology and infectious disease study

✹ Why Choose Blood Agar Base?
- Globally trusted formulation standard in clinical bacteriology for reliable recovery of fragile organisms.
- Eliminates batch-to-batch variation, ensuring uniform plate clarity and dependable hemolytic zones.
- Highly soluble powder boils quickly into a smooth solution without stubborn particulate residues.
- Genuine institutional procurement and dependable stock availability backed by Victor Scientific Co.

✹ Benefits
- Accelerates clinical diagnostic workflows by providing optimal nutritional support for demanding pathogens.
- Maximizes laboratory cost-efficiency through high-yield 500g packaging producing over 12 liters of finished media.
- Reduces contamination risks with rigorous quality control and certified manufacturing standards.
- Backed by certified technical documentation, MSDS, and expert customer support through Victor Scientific Co.

✹ About Victor Scientific Co.
Victor Scientific Co. provides quality laboratory equipment, measuring instruments and scientific tools in Bangladesh. We supply reliable products for educational institutions, industries, laboratories and professional users.

Frequently Asked Questions (FAQ)

Q1

What is the price of Blood Agar Base HiMedia (500g) in Bangladesh?

A1

The price of Blood Agar Base HiMedia M073 (500g pack) in Bangladesh typically ranges between BDT 7,500 and BDT 9,500, depending on import batch and exchange rates. Contact Victor Scientific Co. directly for exact institutional quotes and bulk discounts.

Q2

How do you prepare Blood Agar from this base powder?

A2

Suspend 40.0 grams of Blood Agar Base powder in 1,000 ml of distilled water, heat with frequent agitation to dissolve completely, and autoclave at 15 psi (121°C) for 15 minutes. Cool the medium to 45°C–50°C, aseptically add 5% to 10% sterile defibrinated blood, mix gently, and pour into sterile Petri dishes.

Q3

Can Blood Agar Base be used without adding blood?

A3

Yes, while primarily designed for blood supplementation, the unsupplemented base (Infusion Agar) can serve as a highly nutritious general-purpose growth medium for cultivating less fastidious bacteria.

Q4

What are the three types of hemolysis observed on blood agar?

A4

Beta-hemolysis appears as a clear, transparent zone around colonies due to complete red blood cell lysis; alpha-hemolysis appears as a greenish or brown discoloration due to partial hemolysis; and gamma-hemolysis shows no change or lysis in the agar surrounding the colony.

Q5

Does Victor Scientific Co. deliver culture media safely across Bangladesh?

A5

Yes, Victor Scientific Co. packages all culture media bottles in protective cushioning and moisture-barrier cartons, ensuring rapid courier delivery to all 64 districts in Bangladesh—including Chattogram, Rajshahi, Khulna, and Sylhet—within 24 to 48 hours.

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